Everton midfielder Fabian Delph has withdrawn from the England squad and will miss the Euro 2020 qualifiers in the Czech Republic and Bulgaria.
Delph, 29, picked up a hamstring injury during Everton’s 1-0 Premier League loss at Burnley on Saturday.
A statement from the Football Association said “no further replacements are planned at this moment”.
Boss Gareth Southgate will continue his preparations with a 24-man squad.
England are top of qualifying group A after four wins from four matches and face the Czech Republic away on Friday, 11 October, before playing in Bulgaria three days later.
Goalkeepers: Tom Heaton (Aston Villa), Jordan Pickford (Everton), Nick Pope (Burnley)
Defenders: Trent Alexander-Arnold (Liverpool), Ben Chilwell (Leicester), Joe Gomez (Liverpool), Michael Keane (Everton), Harry Maguire (Manchester United), Tyrone Mings (Aston Villa), Danny Rose (Tottenham), Fikayo Tomori (Chelsea), Kieran Trippier (Atletico Madrid)